Next generation sequencing current technologies and applications pdf

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next generation sequencing current technologies and applications pdf

Next-generation Sequencing: Current Technologies and Applications

It seems that you're in Germany. We have a dedicated site for Germany. In recent years, owing to the fast development of a variety of sequencing technologies in the post human genome project era, sequencing analysis of a group of target genes, entire protein coding regions of the human genome, and the whole human genome has become a reality. Next Generation Sequencing NGS or Massively Parallel Sequencing MPS technologies offers a way to screen for mutations in many different genes in a cost and time efficient manner by deep coverage of the target sequences. This novel technology has now been applied to clinical diagnosis of Mendelian disorders of well characterized or undefined diseases, discovery of new disease genes, noninvasive prenatal diagnosis using maternal blood, and population based carrier testing of severe autosomal recessive disorders. This book covers topics of these applications, including potential limitations and expanded application in the future. JavaScript is currently disabled, this site works much better if you enable JavaScript in your browser.
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GENEWIZ Webinar - Emerging Next-Generation Sequencing Technologies and Applications

Next-generation sequencing technologies and applications for human genetic history and forensics

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Slideshare uses cookies to improve functionality and performance, and to provide you with relevant advertising. If you continue browsing the site, you agree to the use of cookies on this website. See our User Agreement and Privacy Policy. See our Privacy Policy and User Agreement for details. Published on May 3, Ebooks download Next-Generation Sequencing: Current Technologies and Applications by High-throughput, next generation sequencing NGS technologies are capable of producing a huge amount of sequence data in a relatively short time and have revolutionized genome research in recent years. The powerful and flexible nature of NGS has made it an indispensable tool for a broad spectrum of biological sciences and NGS technologies have transformed scientific research in many fields.

Rapid advances in the development of sequencing technologies in recent years have enabled an increasing number of applications in biology and medicine. Here, we review key technical aspects of the preparation of DNA templates for sequencing, the biochemical reaction principles and assay formats underlying next-generation sequencing systems, methods for imaging and base calling, quality control, and bioinformatic approaches for sequence alignment, variant calling and assembly. We also discuss some of the most important advances that the new sequencing technologies have brought to the fields of human population genetics, human genetic history and forensic genetics. Determining the DNA sequence is the most comprehensive way of obtaining information about the genome of any living organism. For decades, Sanger sequencing [ 1 ] using fluorescently labeled terminating nucleotides and electrophoresis has been the gold standard sequencing technology. Sanger sequencing made an early impact in the field of microbial genomics, with the first complete bacterial genome, Haemophilus influenzae , sequenced in [ 2 ].

1. Introduction

Skip to search form Skip to main content. Next generation sequencing: chemistry, technology and applications. The latter aspect will enable the application of genomic knowledge into clinical practice in this and next decades and will profoundly change the diagnosis, prognosis and treatment of many human diseases. It will further demand both philosophical and medical curriculum reforms in the training of our future physicians. View on PubMed. Alternate Sources. Save to Library.

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